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KMID : 0613820120220101371
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Journal of Life Science
2012 Volume.22 No. 10 p.1371 ~ p.1377
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Anti-Fibrotic Effects by Moringa Root Extract in Rat Kidney Fibroblast
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Park Su-Hyun
Chang Young-Chae
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Abstract
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Fibrosis in kidney by internal and external factors causes progressive loss of renal function. Renal fibrosis is the inevitable consequence of an excessive accumulation of the extracellular matrix. TGF-β plays an important role in the process of renal fibrosis and stimulates the synthesis of profibrotic factors, including collagens, fibronectin, and plasminogen activator inhibitor (PAI-1). We examined the effect of Moringa oleifera Lam (moringa) extracts in a rat kidney fibrosis model. We found that moringa root extract suppresses protein expression/mRNA levels of Type I collagen, fibronectin, and PAI-1 induced by TGF-β in renal fibroblasts. Moringa root extract selectively inhibited phosphorylation of TGF-β-induced TβRII and the downstream signaling pathway (e.g., Smad4), and phospho-ERK, but not JNK, p38, or PI3K/AKT. These results suggest that moringa root extract can act against TGF-β-induced renal fibrosis in rat kidney fibroblast cells by a mechanism related to its antifibrotic activity, which regulates expression of fibronectin, Type I collagen, and PAI-1 through TβRII-Smad2/3-Smad4 and ERK. Therefore, moringa root extract is an effective substance for fibrosis therapy and provides a new therapeutic strategy for diseases associated with elevated profibrotic factor synthesis.
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KEYWORD
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Fibrosis, moringa, PAI-1, Type I collagen, fibronectin
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